Western blot analysis of ACVR2A in different various lysates. Lane 1: K562 cell lysate; Lane 2: HepG2 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 58 kDa. Observed band size: 50 kDa. Exposure time: 1 minute;10% SDS-PAGE gel. Primary antibody ACVR2A monoclonal antibody (Product # MA5-50611) with a dilution of 1:1,000 was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG-HRP Secondary Antibody with a dilution of 1:300,000 was used for 1 hour at room temperature. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature.
Supplier Page from Thermo Fisher Scientific for ACVR2A Antibody